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Preparation of Chitosan Nanoparticles and its Incorporation with Lycopene for Antimicrobial Activity
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| Author:
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NOORUL ANEESA.N, PRIYA LOCHANA GAJENDRAN, RADHIKA ARJUNKUMAR, S RAJESHKUMAR
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| Abstract:
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Background: Lycopene is a red-colored, fat-soluble carotenoid, which gives tomatoes and several other fruits their deep red color. Lycopene does not have the pro-vitamin A activity. Chitosan, a natural cationic polysaccharide, is a nontoxic, biocompatible, and biodegradable polymer with multiple pharmaceutical applications including drug delivery and tissue engineering. Nanotechnology or nanoscience refers to research and development of an applied science at the atomic or molecular level.
Aim: The aim of the present study is to assess the antimicrobial activity of the chitosan nanoparticles incorporated with lycopene.
Materials and Method: Lycopene extract was collected and prepared following which synthesis of chitosan nanoparticles was done using 1.0 gram of chitosan was dissolved in 99 ml of distilled water. Characterization of chitosan nanoparticles were done using UV - vis - spectroscopy from 300nm to 700 nm wavelength. Nanoparticle powder was prepared by centrifugation of 8000 rpm for 10min followed by which the antimicrobial activity was tested against Candida Albicans, Streptococcus mutans (gram +), and Lactobacillus sp.
Results: The results show that the Zone of Inhibition (ZOI) of Chito-lyco nanocomposite is demonstrated against bacterial cultures. The antimicrobial activity against candida was greater when compared with S. mutans and lactobacillus at 150µL concentration of lycopene. The antimicrobial activity against lactobacillus was greater when compared with S. mutans and candida at 150µL concentration of chitosan.The antimicrobial activity against lactobacillus was greater when compared with S. mutans and candida at 150µL concentration of Chito-lyco nanoparticles.
Conclusion: The study can be concluded that the Lycopene with increase in concentration has increased antimicrobial activity.Chitosan has similar anti-microbial activity as standard solution in higher concentrations. However, anti-microbial activity of chito-lyco nanoparticles was comparatively lesser than the individual components.Lycopene loaded chitosan nanoparticles may show a great promise for the development of drug delivery system by enhancing the cellular accumulation of lycopene with chitosan. However, further research is necessary to ensure its safety and clinical application.
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| Keyword:
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Antimicrobial properties, Chitosan; Cytotoxicity, Lycopene, Nanotechnology
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP1.432
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| Download:
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