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Bioactive Compounds of Prunus Cerasus L – A Molecular Study
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| Author:
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IMMADI LAXMI SUJITH, SINDHU RAMESH
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| Abstract:
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Introduction: Cherry belongs to the Rosaceae family, and specifically to the genus Prunus. The most common types of Prunus are Prunus cerasus and Prunus avium, the first one is known as sour cherry and the other is called sweet cherry. Sour cherry (Prunus cerasus) is consumed as a nutritional supplement claiming health effects. The aim of the study was to evaluate the bioactive compounds of sour cherry.
Materials and Method: The phenolic compounds and anthocyanin from sour cherry were investigated, a mass of 1 kg fresh sour cherries was first washed in cold tap water and pressed with a laboratory type hand press. The juice expressed was then heat treated at 85? and then cooled to 45?. The samples were stored frozen at –20 ? in glass bottles until used for analysis. Various individual phenolic compounds present in sour cherry were identified and quantified by HPLC and Folin–Ciocalteu method.
Results: The polyphenol content was measured by the Folin–Ciocalteu method and expressed as gallic acid equivalents (GAE). Sour cherry juice contained 9.835 ± 1.092 µg GAE mg-1 of lyophilized juice. Five compounds (ascorbic acid, gallic acid, chlorogenic acid, neochlorogenic acid and cyanidin 3-glucosil-rutinoside) out of different monitored polyphenolic compounds were detected, quantified and confirmed comparing retention times and UV spectra with standards.
Conclusion: The present study describes an optimized extraction procedure for the sour cherry. Sour cherry has a great food-value, when we consider its total phenolic, total anthocyanin, cyanidin 3-glucosylrutinoside, neochlorogenic acid contents.
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| Keyword:
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sour cherry, bioactive compounds, phenolic compounds, HPLC, anthocyanin.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP1.416
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| Download:
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