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Formulation Development of Curcumin Loaded Lozenges: Extensive Characterization and In Vitro Evaluation of Antioxidant Activity, Anti-Inflammation and Antimicrobial Activity
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| Author:
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, MANAS RANJAN SAHOO, M.S. UMASHANKAR, RAMESH R VARIER
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| Abstract:
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Curcuminoid are the natural bioactive polyphenolic compounds with diverse biological activities such as anti-inflammatory, antibacterial, antiviral, anticancer, antioxidant, neuroprotective, hepatoprotective, throat infection, and wound healing activities. However the clinical use of curcumin has been limited due to its poor aqueous solubility and limited gastrointestinal absorption. Hard boiled lozenges (HBL) are attractive dosage form with better therapeutic potential and presentation. The present study focuses on development and characterization of curcumin based hard boiled lozenges from sugar and isomalt. The lozenegs were than evaluated for quality parameters such as hardness, friability, dissolving time and dissolution studies. Characterization of lozenges was done by advanced techniques such as Differential Scanning Calorimetry (DSC), Powder X-ray Diffraction (XRD), Fourier-transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). The stability of curcumin in the lozenges formulation was confirmed using HPTLC, LC-MS analysis and in vitro biological activity such as antimicrobial (E.coli and S.aureus) antioxidant activity (DPPH) and anti-inflammatory activity (COX-1 and COX-2). The XRD, FT-IR and DSC results indicates partial transformation of curcumin into its amorphous form in the HBL formulation. HPTLC and LC-MS analysis indicates the curcumin is stable during hot-melt-molding (HMM) process without any substantial interaction between curcumin and the lozenges matrix. The in vitro bioactivity study showed that formulation does not affect the in vitro antimicrobial, anti-inflammatory and DPPH activity in the final products. Overall, the interesting finding of this study suggested that curcumin lozenegs can provide attractive and alternative platform to develop new formulation for existing phytochemicals and other active pharmaceutical ingredients.
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| Keyword:
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Curcumin, Hard -lozenges, Formulation, Solid dispersion, Characterization.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP2.310
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| Download:
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