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The Anti-cancer Activity of Indonesian Marine Sponge Aaptos suberitoides Extract on Colon Cancer Cells
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| Author:
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MUHAMMAD HASAN BASHARI, MUHAMMAD RAFLY SAMODRO, WAHYU SYAHRUL RAMADHAN, DANY M. DAFFA, ANNISA DEWI NUGRAHANI, BEGINER SUBHAN , HAROLD ATMAJA, TENNY PUTRI, NURUL QOMARILLA, HERMIN AMINAH USMAN, EKO FUJI ARIYANTO
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| Abstract:
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Purpose: This study aimed to evaluate anti-cancer properties of Aaptos suberitoides in cytotoxic activities,
proliferation and cell migration of Caco2 and HCT116 colon cancer cells.
Methodology: The sponge Aaptos suberitoides were collected from Pramuka, Tinjil and Tidung Island, Indonesia.
Samples were extracted with ethanol. To evaluate its effects, Caco2 and HCT116 cells were exposed to this
extract for cytotoxic assay using MTT, antiproliferative effect using clonogenic assay, and cell migrations using
scratch assay. Determination of IC50 is done by analyzing cell death and area of colonies data using fourparametric
logistic using Sigmaplot ver.12, and to determine the area of closure gap area is done by using
ImageJ software.
Results: The initial bioscreening results showed that the extract of Aaptos suberitoides from around Tinjil Island
has the strongest cytotoxic effect on Caco2 cells compared to other samples. Furthermore, the results
showed that the cytotoxic IC50 of this Aaptos suberitoides extract was 8 ppm and 11 ppm in Caco2 and
HCT116 cells, respectively. Moreover, it also showed anti-proliferative in Caco2 with IC50 7 ppm. In addition,
this extract also inhibit cell migrations in low concentrations.
Applications/Originality/Value: Aaptos suberitoides extract caused death, inhibits proliferation and cell migration in
colon cancer cells.
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| Keyword:
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CT116, MTT
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| DOI:
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