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The potential use of Medang-medangan (Endiandra rubescens Lauraceae) extracts as antioxidant and anticancer agents
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| Author:
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GALUH WIDIYARTI, RIZNA TRIANA DEWI, SRI HANDAYANI
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| Abstract:
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Purpose: In this research, we studied the potential use of leaf and stem bark extracts from medang-medangan
(Endiandra rubescens Lauraeae) that one of the endemic plants of Indonesian tropical forest as antioxidant
agents. Toxicity of the extracts was also studied to investigate anticancer potential of the extracts.
Cytotoxicity of the extract that most active as an antioxidant was carried out against human breast cancer
MCF7 cells to know the possibility as anticancer agent candidate of the extract.
Methodology: Extraction of medang-medangan was conducted by maceration method using methanol, and then
the methanol extract was partitioned using n-hexane and ethyl acetate, successively. The methanol extract was
then preliminary phytochemical screened to qualitatively determine the presence of different classes of
secondary metabolites. All extracts were then determined for their total phenol content (TPC) and total
flavonoid content (TFC) using spectrometric method. 1-diphenyl-2-picryl hydrazyl (DPPH) radical method was
used to determine antioxidant potential of the extracts, while Brine Shrimp Lethality Test (BSLT) method was
used to analyze toxicity of the extracts. Extract that most active as antioxidant was then cytotoxic activity
tested aginst human breast cancer (MCF7) cells using alamar blue method.
Results: The preliminary phytochemical screening indicated the presence of alkaloids, flavonoids, tannins,
saponins, terpenes and quinones of the extracts. All extracts were very active as antioxidant with IC50 value of
1.61-6.69 µg/ml which their antioxidant activity about 2.1 up to 2.8 times than antioxidant activity of ascorbic
acid as a standard antioxidant. Methanol extract of leaf has highest antioxidant activity due to its TPC (306.25
µg GAE/mg of extract) and TFC (52.82 µg QE/mg of extract) was highest than the other extracts. All extracts
were categorized as mildly toxic with LC50 value of 56.24–390.04 µg/ml. Methanol extract of leaf has also
cytotoxic activity against MCF7 cancer cells with IC50 value of 80 µg/ml. The medang-medangan extract
potentially to be developed as natural antioxidant and possibility as anticancer agent candidate.
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| Keyword:
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Medang-medangan, TPC, TFC, antioxidant, toxicity, anticancer.
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| EOI:
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| DOI:
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