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Antituberculosis assay in-vitro of ethanolic, n-hexana, chloroform, ethyl acetate extract of Cordyline fruticosa (L) A.Cheval leaves
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| Author:
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CUT FATIMAH, MELATI, HARRIS
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| Abstract:
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Purpose: According to WHO has identified so much people with tuberculosis disorder, and includes a disease
that causes death. Mycobacterium. tuberculosis has been resistant to the synthetically antituberculosis drugs.
Traditionally Cordyline fruticosa (L) A.Cheval leaves (andong in Indonesia) used to treat cough and bloody
sputum. This study conduct to investigate the potency of ethanolic, n-hexana, chloroform, ethyl acetate
extract of Cordyline fruticosa (L) Chev as anti-tuberculosis in vitro.
Methodology: The extract of Cordyline fruticosa (L) A.Cheval leaves was prepared by percolation using
ethanol, and fractination using n-hexana, chloroform, ethyl acetate as solvent. Mycobacterium tuberculosis
was isolated, identification and cultivated from bronchial fluid (sputum) speciments tuberculosis disorder in
Pulmonary Disease Treatment Centre Medan Sumatra Utara. Antituberculosis assessment examination
conducted by Mycobacterium tuberculosis of ethanolic, n-hexana, chloroform, ethyl acetate extract of
Cordyline fruticosa (L) A.Cheval leaves was performed at concentration of 200mg/ml, 100mg/ml, and 50mg/ml
by Lowenstein-Jensen (LJ) method against Mycobacterium tuberculosis, compared to commercial preparation
of Rifampicin 40µg, Ethambutol 10µg, Isoniazida 0,2 µl.
Result: Ethyl exacetate extract of the Cordyline fruticosa (L) A.Cheval leaves could inhibit the growth of
Micobacterium tuberculosis, useful as an antituberculocis compared to extract of ethanol, n-hexana,
chloroform, ethyl acetate extract 100 mg/ml exhibited a strong inhibition against the growth of Mycobacterium
tuberculosis from sputum of tubercolosis disorder similarly to Rifampicin 40µg, Ethambutol 10 µg, and
Isoniazida 0,2 µg commercial preparation.
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| Keyword:
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Cordyline fruticosa (L) A.Cheval leaves, antituberculosis, Lowenstein-Jensen
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| EOI:
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| DOI:
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