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Assessment of Kisspeptin (metastin) hormone and Genetic Aspect of Polycystic Ovary Syndrome Women in Thi Qar Province, Iraq
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| Author:
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, HAIDER SADIQ MUTASHER, ENAS ABDUL KAREEM JABBAR
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| Abstract:
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Background: Polycystic ovary syndrome (PCOS) is a complex heterogeneous disorder with multifactorial etiology (endocrine, genetic, metabolic and environmental factors). Most women with PCOS suffer from menstrual irregularity, hirsutism, alopecia, acne, infertility, alongside several metabolic disorder such as insulin resistance, obesity, hypertension and hyperlipidemia.
This study was designed to estimate kisspeptin ( metastin ) hormone , follicle-stimulating hormone (FSH), luteinizing hormone(LH) and testosterone levels in women with polycystic ovary syndrome (PCOS), in comparison with controls, and study the correlations between kisspeptin and PCOS-related reproductive hormones, and investigate the relation between probable influence of KISS1 gene polymorphisms and hormone levels in women suffering from PCOS
Methods: The study comprised 100 PCOS women and 100 controls(healthy women), with age ranging from 17 to 40 years. Blood samples were collected between day 3 and day 5 of the menstrual cycle(follicular phase) in both groups, BMI and circulating levels of serum kisspeptin(metastin), LH, FSH and testosterone were estimated. DNA was extracted from whole blood, and genotyping of rs4889 polymorphism in KISS1 gene was carried out by allele-specific PCR.
Results: The values of BMI, kisspeptin, testosterone LH and LH-FSH ratio were significantly higher in PCOS women than controls. Plasma kisspeptin levels were positively correlated with LH levels. There was no statistically significant difference between the groups in terms of FSH level.
The SNP rs4889 C/G, a non-synonymous SNP, was investigated in women with PCOS. The genotype (CG) had a significantly higher frequency (P=0.004) in PCOS group with OR of (2.316)than that of the control group. This would reflect that the genotype(CG) is a risk factor for PCOS occurrence within the sample size. Concerning the allele frequency of rs4889, the allele (C) showed a significantly higher frequency (41.5%, P=0.0008) in the PCOS group than that of the control group (25.5%).
Conclusion: Overall, kisspeptin levels are higher in the PCOS women, this may be because disorder in hypothalamic-pituitary-gonadal(HPG)axis activity, thereby causing an increased level of LH which lead to irregular menstrual cycles and hyperandrogenism in PCOS women. Also, the results of the study show that the KISS1 gene polymorphism may be a factor contributing to PCOS development by its effect on increasing the kisspeptin hormone in the blood. The association between the gene and the gene variation and PCOS need further validation in large-scaled and functional studies.
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| Keyword:
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Kisspeptin , metastin , KISS1 gene , PCOS
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP2.168
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| Download:
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