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The combination of pioglitazone overdose has been associated with an increased risk of a CYP1A1 gene for bladder cancer in male rats
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| Author:
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, ZAHIDA MIRAN HUSSEIN ALQAYYIM
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| Abstract:
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This study aims to highlight the urinary bladder tumor, currently one of the most common types of human cancer throughout our planet. Several predisposing factors play an important role in causing this tumor, including both internal and external blame. The polymorphism of the CYP1A1 gene was recently linked to the occurrence of bladder cancer. Pioglitazone, which has been used to treat type II diabetes for a long time, has a serious effect on the induction of this tumor.
The aim of this study was to assess the risk of using pioglitazone in male mice who had CYP1A1 polymorphism to induce bladder cancer.
MATERIALS AND METHODOLOGY: This study was conducted by 90 rats, where 3 groups were injected with pioglitazone for type 2 diabetes mice (3,6,12mg / kg body weight), respectively, and they were evaluated in the case of using pioglitazone with the presence of forms Multiple CYP1A1 associated with a higher risk of CA bladder. DNA was extracted and molecular detection was performed to detect single polymorphism (SNPs) at genetic sites using PCR and PCR-RFLP techniques.
RESULTS: There was an additional risk of long-term use of pioglitazone at group V high dose of 12 mg / kg bw pioglitazone in the occurrence of CA bladder in male rats with polymorphism CYP1A1 significant increase p<0.05 with group (I) , Group III, IV less increase with control group I,II.
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| Keyword:
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CA bladder, pioglitazone. CYP1A1 polymorphisms
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP2.163
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| Download:
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