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Frequency of micronuclei and apoptosis in exfoliated buccal cells in chronic inflammation of oral mucosa
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| Author:
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FADIL A.AL-QURAISHE, IHSAN ABDULLAH KUMAIL
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| Abstract:
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Chronic inflammations of the oral mucosa (CIOM) induced by biological , chemical , and physical factors and are in turn associated with an increased risk of oral cancers . The objective of the current study was the investigate the spontaneous genetic damage using the micronuclei (MN) and apoptosis test on exfoliated cells from (CIOM) patients.
Cytological tests included , in addition to (MN) , counting apoptosis ( karyorrhexis , condensed chromatin , and , pyknosis). The study sample comprised 42 individuals aged ( 20 – 40 ) years including twenty two patients( 13 males and 9 females ) revealed (CIOM) and the another 20 ( 12 males and 8 females ) were healthy control group . MN frequency was significantly increased (P<0.001) in buccal mucosa cells obtained from lesion area (LA) and normal area (NA) from chronic inflammation patients with mean value (7.53 ± 1.49) and ( 5.02 ± 1.47 ) respectively, when compared with the healthy control group ( 2.40 ± 1.40) . Lower frequency of apoptosis observed in ( LA ) from chronic inflammation patients compared with control group ,was (P = 0.06) more over apoptosis was significant increased (P<0.001) in NA of chronic inflammation patients when compared with cells of control group .The MN was regarded with the gender , age , mouthwashes uses , and , oral hygiene of the patients . The frequency of MN was significantly higher in the females than males in both patients and control groups ( P < 0.01), whereas non-significant differences in MN occurrence was observed in relation to age.
However MN frequency was significantly higher in poor oral hygiene and mouthwash users(P<0.01) patients group but shown non-significant differences in poor and good oral hygiene in control group . Also the significantly higher frequency was increased MN in mouthwashes user in control group, when compared with non-users . The results suggest that increased MN and decreased apoptosis in the long periods chronic inflammation of the oral mucosa patients could be consider as a useful diagnostic bio- monitoring assay to prevent the risk from transformation of chronic inflammation to pre cancers lesions.
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| Keyword:
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Micronuclei , Apoptosis , Chronic inflammation , Oral mucosa exfoliated
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP2.153
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