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Article Detail
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Molecular virulence determinants and Antimicrobial susceptibility patterns of Pseudomonas aeruginosa isolated from Clinical infection patients in Al Diwaniyah city of Iraq
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| Author:
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, HASSAN HACHIM NASER, ADNAN H. AUBAID
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| Abstract:
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The study was comprised collected 137 Pseudomonas aeruginosa isolates which isolated from sputum, burns, urine, and ear swab clinical patients samples. All collected Pseudomonas aeruginosa isolates were confirmed by selective Pseudomonas Chromogenic agar and biochemical identification by Vitek 2 system. The antibiotic susceptibility patterns by using disc diffusion method according to (CLSI guide 2018). the results were showed high frequency of MDR isolates at 101 (73.72%) and less frequency of XDR isolates at 16 (11.67%) and no isolates were recorded as pandrug antibiotics resistance (PDR) with High Significant differences (P<0.01). The burns isolates were showed almost high frequency of MDR isolates at 38 (97.37%) followed by urine, sputum, and ear swab isolates at (62.50% , 60.90%, and 72.0%). The frequency of XDR isolates were showed at (28.9% , 8.0% , 8.0%, and 3.12%) in burns, urine, sputum, and ear swab isolate respectively, with High Significant differences (P<0.01). All XDR Pseudomonas aeruginosa isolates were showed non susceptibility to Piperacillin, Amikacin, Netilmicin, Imipenem, Meropenem, Ceftazidime, Cefepime, , and Aztreonam at (100%) . Other XDR isolates were showed less susceptibility to Ofloxacin, levofloxacin, and Gentamicin at (56.25%, 56.25%, and 75.0%) whereas, no XDR were showed resistance to Colistin and polymyxin B. The biofilm formation assay results were showed most XDR Pseudomonas aeruginosa isolates given strong biofilm formation at prevalence (81.25%). The XDR Pseudomonas aeruginosa isolates were genetic confirmative detected by PCR based 16S ribosomal RNA gene. The PCR was showed almost XDR Pseudomonas aeruginosa isolates have ToxA, exoU, exoS virulence factors genes at (87.5%, 100%, and 100%) respectively. The biofilm formation was also detected at (100%) algC and (93.75%) lasI gene. In conclusion, Pseudomonas aeruginosa is important causes of nosocomial diseases and has high ability to acquired multidrugs resistance capacity especially in burn infection patients, and almost XDR Pseudomonas aeruginosa isolates has potential virulence factors with high ability to biofilm formation with nonsusceptibility to almost antimicrobial drugs agents except lipopeptides agents.
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| Keyword:
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Pseudomonas aeruginosa, virulence factors, and antibiotic resistance.
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP2.152
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| Download:
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