Phenotypic and genotypic characterization of Acinetobacter baumannii Iraqi isolates
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Author:
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, ASMAA MOHAMMED MEKKEY, SHAIMAA A. AL-OUBAIDY, JAWAD RASHED ALZAIDI
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Abstract:
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Biochemical and microscopic tests (that identified the phenotypic characterization) and Genotypic detection of Acinetobacter baumannii is an important health care issue. The isolated A. baumannii from various clinical specimens, were identified according to microbiological, biochemical and molecular tests (amplification of organism specific blaOXA-51 and VIM genes). In a present study (85) clinical isolates from different clinical sources including (Sputum, Wounds, Burns, UTI, Blood). (70) samples were correctly identified according to Api 20 test and VITEK 2. PCR was performed (70) samples and the PCR result was (33) samples by using OXA-51 gene detection. This study evaluated the accuracy between the VITEK 2 system, API 20 NE system and genotypic detection methods.
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Keyword:
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Phenotypic, genotypic, isolates
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EOI:
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-
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DOI:
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https://doi.org/10.31838/ijpr/2020.SP2.148
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Request For Article
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