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Isolation and Antimicrobial susceptibility Profile Shigella spp. from children under five years with diarrhea in Al-Nassiriyah City
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| Author:
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, SERAJ SADIQ HUSSAIN, EZAT HUSSAIN MEZA, MOHAMMED HASHIM AL-YASIRI
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| Abstract:
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The present study aimed at isolation and identification of Shigella spp. from human children with diarrhea and characterization of the samples using biochemical and molecular characterization techniques. our study was carried out during the period from November 2017 to May 2018. 400 fecal samples were collected from children aged (1day-5 years) of both sexes (216 Male, 184 Female) that had suffering from diarrhea in Mohammed Al -Mosawi Hospital and public health lab. in Al-Nassiriyah City. fifteen isolates (3.75%) were diagnosed as genus Shigella by using API 20E , VITEK2 systems and molecular detection by using ipaH gene. Antimicrobial susceptibility testing done by using 9 Antibiotics from different classes showed that all isolates were resistance to Ampicillin and Nalidixic acid, however all were sensitive to Chloramphenicol, resistance percentage to Cefotaxime, Gentamicin, Amikacin, Ceftriaxone, Ciprofloxacin and Tetracycline at (40%), (40%), (33.3%), (33.3%), (26.6%) and (20%) respectively.
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| Keyword:
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Shigella spp., antimicrobial susceptibility, ipaH
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP2.146
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| Download:
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