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Development and validation of hydrophilic interaction chromatography for determination of deoxyadenosine in human serum
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| Author:
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ASHRAF SAAD RASHEED, AMEEN WALEED QASSIM, SALSAL KAMAL ABDULRAHMAN
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| Abstract:
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For deoxyadenosine determination, a simple ZIC-HILIC-UV technique was established and applied to the hu-man serum. The separation was carried out by gradient elution mode with acetonitrile acetate buffer and UV detection of 254 nm, on ZIC2 and ZIC5 self-constructed columns. The deoxyadenosine behavior with various acetate buffer, ACN proportion, and pH values was examined and the findings confirmed the deoxyadenosine hydrophilicity. The separation mechanism is premised on the partitioning of the deoxyadenosine in the hydro-philic and ion exchange. We have defined and mentioned the current effect of chromato-graphic circumstances (sodium acetate buffer concentration, ACN, and the pH) for both ZIC1 and ZIC5 columns. The two existing methods are a useful alternative to current separation methods for deoxyadenosine.
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| Keyword:
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deoxyadenosine, ion exchange, nucleoside, ZIC- HILIC, hydrophilic interaction
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP2.050
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| Download:
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