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The impact of moderate Hypoxia on cytokines-driven beta cell apoptosis
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| Author:
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BUTHAINAH AL-AZZAWI, AJILE ALZAMILY, CATRIONA KELLY, NICK FORSYTH
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| Abstract:
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Background: Hypoxia displays different natural impact which could be either harmful or valuable. It is accepted that pancreatic islets cells are profoundly touchy to hypoxia conditions. Hypoxia could bring about speeding up of beta cells passing in diabetes mellitus type one. The secretion of pro-inflammatory cytokines could encourage the obliteration of pancreatic beta cell. However, the connection among hypoxia and cytokines-driven beta cell apoptosis is indistinct.
Methods: The reaction of two beta cell lines and essential islet cells to the expansion of explicit cytokines was assessed for both normoxia (21%O2) and Hypoxia (10%O2) condition through MTT test, apoptosis was guaranteed utilizing TUNEL test and qPCR was done to search for the hereditary contribution.
Results: The results showed that the viability of beta cell lines and primary islets cell were reduced after the addition of cytokines. However, the viability of cells grown under 21%O2 condition was csignificantly (P<0.05) higher as compared to those cultured in 10%O2 condition. A20 and TRAIL gene expression were significantly (iP<0.05) higher in cells treated with cytokines for both 21%O2 and 10%O2 culture condition.
Conclusion: The moderate decrease in oxygen level may increase the sensitization of pancreatic beta cell to the cytokines addition, the viability of beta cell grown under this moderate 10%O2 was significantly lower than those grown under 21%O2 condition.
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| Keyword:
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apoptosis, beta cell, 10%O2, insulin, diabetes mellitus type one.
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP2.049
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| Download:
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