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Article Detail
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Diagnostic importance of circulating microRNA (23a, 451a) in multiple sclerosis
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| Author:
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IBRAHIM A.ALTAMEMI, HAYDER K.HASSOUN, DHAFER T.ALWADEES
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| Abstract:
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Background: In adults as young, sclerosis as multiple considered as most cause common of disability as neurologic. MS is characterized by demyelination, inflammation, and injury of neuro-axonal at nervous central system causing long-term developing disability. At the current time no definitive single exam for MS diagnosis and monitoring.
A big challenge in (MS) is to biomarkers developing which might helping to understand MS patients as individual, such if they are a therapy responder or not, that medicine is effective more, as well as degree to that they might entering disease developing phase. At last years, huge attention has drawn to process-specific, biomarkers treatment-related diagnostic, and prognostic for MS identification. At the current review, we are concentrating on candidate’s potential as neurofilaments for biomarkers of MS.
Objective: The aim of present study is to find out a non-invasive diagnostic miRNA biomarker (miRNA-23a, miRNA-451a) for multiple sclerosis with high sensitivity and specificity.
Patients and methods: A case control study based on three groups. first group 48 patients with previously diagnosis as MS and under treatment which include (11 male and 39 female), also second group was 20 patients new cases without treatment or who have sign of MS or suspected MS which include 7 male and 13 female who were observation in Middle-Euphrates-Neuroscience-Center in Anajaf Al-Ashraf/ Iraq. Third group was include 20 healthy volunteers (non-MS) . Samples of blood (5 ml) were taken via venipuncture out of venous from such groups drawed via syringes being disposable at technique of aseptic. Every sample of 3 groups were taken in free plane EDTA tube, permitted for clotting then separation to serum was done via centrifuge of 13000 rpm for 5 min and then stored at -20C.Which was further used to identify miRNA-23a and miRNA-451a qPCR.
Result: levels of miR-451a and miR-23a for MS patients and healthy individuals are shown in table 3.11 and figures 3.5 and 3.6. High difference of significe in miR-451a between groups, the level in control group was constant and equals one fold change; the level of both groups of patients of higher compared to healthy individual group, but, difference of no significant in the level between both patients groups (P > 0.05)
Added to that, there was high difference of significance in miR-23a between study groups; the level in control group was constant and equals one fold change; the level of both patients groups was higher than that of control group (P < 0.05), but, there was no significant difference in the level between both groups of patients (P > 0.05).As soon as the analysis of ROC curve was done and outcomes showed that ,the miR-451a cutoff value was >1 fold change with AUC,95%CI, sensitivity, specificity and accuracy level 0.721, 0.615 to 0.811,72.1%, 100% and 72.1% respectively. While the miR-23a cutoff value was >1 fold change with AUC,95%CI, sensitivity, specificity and accuracy level 0.735, 0.630 to 0.824, 73.5%, 100% and 73.6% respectively.
Conclusion:We can get a diagnosis biomarker with high sensitivity and specificity, by using a combination of these two miRNA. miRNA 451a which show high sensitivity, and miRNA23a which show high sensitivity (72.1 and 73.5) respectively, and AUC (0.721 and 0.735) respectively. This could possibly complement one another in an indicative test while enhancing sensitivity when related to the miRNAs individually.
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| Keyword:
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Diagnostic, sclerosis.
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP2.048
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