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In-vitro Evaluation of Metronidazole loaded Mesoporous Silica Nanoparticles Against Trichomonas Vaginalis
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| Author:
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DHIYA ALTEMEMY, MAHNAZ JAFARI, KOUROSH MANOUCHEHRI NAEINI, SHAIMAA ALSAMARRAI, PEGAH KHOSRAVIAN
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| Abstract:
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Mesoporous silica nanoparticles (MSNs) are known as carriers with high loading capacity and large functionalizable surface area for more efficient drug delivery. In this study, the efficacy of metronidazole-loaded mesoporous silica nanoparticles (MSNs/MTZ) with a pH-sensitive release against Trichomonas vaginalis trophozoites was investigated. MSNs were prepared by the sol-gel method and final particles were characterized by Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Brunauer Emmett Teller (BET), Fourier Transform Infrared (FT-IR) spectroscopy and X-ray Diffraction (XRD) methods. MSNs were loaded with metronidazole (MTZ) to MSNs/MTZ preparation. In vitro susceptibility assay of MTZ/MSNs in Trichomonas vaginalis trophozoites was done to obtain inhibitory concentration (IC50). SEM results show particles with an approximate size of 50 nm and XRD results prove the crystalline structure of MSNs. The BET results show that pore size and surface area of these particles are 2.8 nm and 800 m2/g respectively. Other FTIR results confirm the chemical structure of the nanoparticles. The loading capacity of MSNs was 33% and the release of MTZ from the nanoparticles affected by the pH. Toxicity assay results on Trichomonas vaginalis trophozoites show increased cytotoxicity in MSNs- MTZ compared to free MTZ, whereas this evaluation confirmed that MSNs toxicity was insignificant. According to the results, it can be said that the use of MSNs is a good option for drug delivery to Trichomonas vaginalis trophozoites, and the use of these nanoparticles make MTZ more effective and performable.
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| Keyword:
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MSN, MTZ
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP1.400
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| Download:
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