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In-Vitro Biological Activity of Areca catechu Extract.
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| Author:
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SAMRIN H.NADAF, ANJALI V. SALUNKE , SUKANYA U. YANDE, VAISHNAVI V. PATIL, AARTI H. GHODKE, AJAY KUMAR OLI
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| Abstract:
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Areca nuts (Supari) are the seeds from the fruit of the Areca Catechu Palm tree. Areca catechu L. (Palmae), cultivated in subtropical and tropical region in India. It is an economically important seed crop. They are often referred to as betel nuts as they are typically chewed with leaves from the betel plants. It is used as a folklore medicine for the treatment beriberi, constipation and oedema. It is also reported that it possesses anthelmintic, anti-inflammatory, antioxidant, psychoactive, antidepressant and anti-HIV. In our present study the locally available A. catechu were collected, pre-sterilized and the abstraction of metabolites was conceded out by soxhlet apparatus method. Areca nuts extract were processed for phytochemical and biochemical analysis. Anti-cleavage property, lethal assay on shrimp and In-vitro assay on cancer cell line was performed. The results reveal the presence of the secondary metabolite terpenoids in all used solvents systems. The toxicity study was carried out using In-vitro model (shrimps) showed less cytotoxicity. The MTT method indicated that extract of areca nut present significant reduction in inhibition of cell proliferation of cell line A431 and CHOK-1. It can use as rapid, simple and economical substitute for cytotoxicity assays. It also correlates with other In-vivo, In-vitro cytotoxicity assays. In-vivo and In-vitro cytotoxicity assays. Advantage of using In-vitro model is to reduce the usage of animals. It also helps to compare the range of concentrations used for expected In-vivo studies. The metabolite extracted can be used as bio-agents or therapeutic drugs for the treatment of life threatening diseases.
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| Keyword:
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A. Catechu, Areca nut, betal nuts, MTT assay, cytotoxicity, brine shrimps
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP1.398
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