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GC-MS profile, antibacterial, antifungal and anticancer activity of leaves and roots of Vanda testacea an epiphytic orchid
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| Author:
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N.V.RAMANA , R. NEELIMA, RATNA KUMAR P.K, B.V. RAO
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| Abstract:
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The presence of phytochemical constituents has been reported from species of the Orchidaceae. Hitherto no reports exist on the antibacterial, antifungal and anticancer activity and GC-MS Profile of Vanda testacea which was an epiphyte from the sacred groove of Srisailam of Andhra Pradesh. The current study was to determine the antibacterial, antifungal, anticancer activities, and GC-MS profiles of the leaves and roots of Vanda testacea.
The test material was the leaf and root of the Vanda testacea which was an epiphytic orchid from the sacred groove of Srisailam of Andhra Pradesh. An in vitro evaluation of antibacterial and antifungal activity by disc diffusion method and dilution technique, and anticancer activity by SAB assay. Gas chromatography-mass spectrometry (GC-MS) analysis of the effective leaf and root extracts of Vanda testacea was performed on GC-MS equipment (GCMSQP2010, SHIMADZU).
The GC-MS profile has revealed the presence of different phytochemical compounds in the leaf and root extracts of Vanda testacea. A total of 19 compounds were identified. The finding of the majority of antiviral compounds with GC-MS Peak area% 71.1% and 27.4% Dimethyl sulphide in methanolic leaf and root extracts, similarly 99.94% and 99.88% of Di-malic disodium salt in ethyl acetate leaf and root extracts indicated the antiviral nature of the species. Furthermore, the results of antibacterial and antifungal tests (both primary and secondary) shown antibiotic activity, and the anticancer activity results are shown inactivity.
From the results, it is evident that bioactive compounds present in the leaf and root extracts of Vanda testacea has shown strong antibacterial, and antifungal activity confirmed the pharmaceutical applications of the leaves and roots. GC-MS profile of leaf and root extracts revealed the presence of a majority of antiviral compounds proven that species has potent antiviral properties.
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| Keyword:
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Epiphytes, Orchidaceae, antibacterial activity, antifungal activity, methanol, n-hexane and ethyl acetate, Disc diffusion method, Dilution technique, SAB assay, GC-MS analysis.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP1.333
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| Download:
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