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Antioxidant and Anticancerous Activities of Biologically Synthesized Zinc Oxide Nanoparticles (ZnO NPs) from the Rhizomes of Curcuma longa
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| Author:
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VINOY JACOB, P. RAJIV, SUGAPRIYA DHANASEKARAN
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| Abstract:
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Curcuma longa, a wide spread perennial herbaceous plant with enormous amount of bioactive compounds in its rhizome, can serve as an ecologically and economically favourable template for the green synthesis of the Zinc Oxide Nanoparticles (ZnO NPs). Zinc with its multifunctional properties can act as a toxic factor or activating factor depending upon the environment, concentration, physical and chemical properties. In this regard, the investigation deals the assessment of antioxidant and anticancer properties for C. longa rhizome mediated ZnO NPs. Bio-synthesized ZnO NPs from C. longa were found to be spherical (average size: 25 nm). Different concentrations of ZnO NPs were employed to assess the antioxidant activity by various biochemical assays. All the antioxidant assays were proved to be positive with the activity being highest at a concentration of 175 µg/ml. Moreover, the cytotoxicity studies have been carried out in human breast cancer model cell line Michigan Cancer Foundation-7 (MCF-7) using trypan blue-dye exclusion assay, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Lactate Dehydrogenase (LDH) assay, colony forming assay, nitrite determination assay and caspase activity assay. MTT assay clearly showed that an IC50 value of 47 µg/ml ZnO NPs prevent the growth of human breast cancer cells.
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| Keyword:
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Curcuma longa, Zinc Oxide Nanoparticles, Antioxidant, Cytotoxicity, MCF-7 cells, Anticancer activity
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP1.283
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| Download:
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