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Optimization and validation of a rapid and direct sampling method for the estimation of lead in whole blood using graphite tube atomizer- atomic absorption spectroscopy
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| Author:
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RAVENDRA VERMA, SWATI MADAN, FARAAT ALI , SATYENDRA K. RAJPUT
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| Abstract:
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An extensive validation of a rapid and direct sampling method was done for estimation of lead content in whole blood
using graphite tube atomizer atomic absorption spectroscopy without pre-digestion. The sequence of sample
introduction and amount of addition of a mixed matrix modifier solution is conceded here. The current method is
optimized and rigorously validated within the commonly disseminated lead toxicity range on whole blood samples
without a tedious step of sample preparation and/or digestion. Multivariate optimization and validation were used to
establish the optimal conditions of the graphite tube atomizer and matrix modifier solution for blood lead detection.
No significant differences were found between the results obtained using the proposed method and the optimized
mixed matrix modifier solution for the lead analyte estimation in whole blood matrix.
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| Keyword:
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Lead, Graphite Tube Atomizer Atomic Absorption Spectroscopy, Direct sampling, Whole blood, Validation.
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| EOI:
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| DOI:
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| Download:
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