|
Synergistic Effect of Photobiomodulation and Viscum album Extract on Colorectal Cancer HCT-116 Cell Line
|
|
| Author:
|
ZAINAB A.HADI, RANA A.GHALEB, NISREEN J.MOHAMMED
|
| Abstract:
|
Background and Objectives: Photobiomodulation-based therapy, a process that causes biological changes in organisms due to interaction with atoms or molecules by photons. Viscum album is often prescribed to improve many health issues. white-berry mistletoe extracts have been used in complementary and alternative cancer therapy in Europe. The aim of this in vitro study was to analyze the effect of a green laser (?= 532nm, Power= 286mW), Viscum album, and their combination on the viability (cytotoxic or enhancement) of colorectal cancer HCT-116 cell line after 24, 48 and 72 hours of incubation periods.
Materials and Methods: Colorectal cell plates (HCT-116 cell line) grown in culture medium supplemented with 10% fetal bovine serum (FBS) were irradiated with a green laser, treated with the Viscum album extract, and treated with the combination of them and plates incubated for 24,48, and 72 hours respectively. Crystal violet assay used to determine the viability of the cells in all the experiments, and the intensity of color was measured by plate reader.
Results: Green laser results showed a considerable decrease (P=0.001) after 48 and 72 hours incubation time in the viability percent, Viscum album extract appeared a considerable decrease in the viability percent (P=0.001) for all concentrations and the most effective concentration was 1000 µg/ml, and the combination results showed a significant decrease in cell viability percent (p=0.001) for all concentrations as compared with the control group.
Conclusion: It was concluded that the higher concentration (1000µg/ml) of V. album had a better anticancer effect on HCT-116 cell line for 24,48 and 72 hours. The laser had a better anticancer effect after 48 and 72 hours. The combination results had a synergism effect by reducing the cell number more than using the Viscum album or a green laser separately.
|
| Keyword:
|
Photobiomodulation, Viscum album, HCT-116 Cell Line
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2020.SP1.303
|
| Download:
|
Request For Article
|
|
|