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Computational analysis and modelling of outer membrane protein A of Acinetobacter baumannii
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| Author:
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WATHIQ ABBAS HATITE, BAKR MOHAMMED ALI ABDUMOHSIN
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| Abstract:
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The outer membrane protein A of A. baumannii (OmpA) is the most abundant surface protein in A. baumannii and have been considers as multi-virulence factor. computational tools and servers have been used to analysis the amino acid sequence of OmpA protein and predict the three dimensional (3D) structure of OmpA , the predisi and SignalP-5.0 servers were agreed that protein sequence contain a signal peptide 1-22 amino acid and the cleavage accord between amino acid 22-23 also the Physicochemical properties of OmpA sequence were obtained using Protparam server, I-TASSER and RaptorX servers were using to predict OmpA structure and according to validation step do by RAMPAGE: Ramachandran Plot Assessment and PROSA servers the model predicted by RaptorX server was better than I-TASSER server’s model . Finally refinement step by 3D Refine server has not improved the model quality that make the structure model before the refinement more confidence
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| Keyword:
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I-TASSER, RaptorX, A. baumannii, OmpA, PROSA, RAMPAGE: Ramachandran Plot Assessment,
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP1.313
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| Download:
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