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Preparation, Characterization and Biological Test of Biodegradable Composite Tissue Engineering Scaffold
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| Author:
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P.MUTHU , S.P.ANGELINE KIRUBHA
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| Abstract:
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We report fabrication of electro-spun polycaprolactone (PCL), gelatin and PCL–gelatin scaffolds in tissue
engineering based applications. The structural characterizations were investigated by fourier transform infrared
spectroscopy (FT-IR) and scanning electron microscopy (SEM) for the fabricated scaffolds. Pure PCL and
gelatin scaffolds showed 3D nano-fibrous porous structures at 20 wt.% and 30 wt.%, respectively. PCL-gelatin
scaffold at 70:30 vol.% showed good fibrous morphology without any structural discontinuities such as polymer
beads, droplet formation and uneven fibers. Cellular activities such as cell adhesion and growth on scaffold
surfaces were performed with F11 human fibroblast cells via MTT assay. Cell viability study showed that
PCL-gelatin scaffold significantly improved the cell adhesion and proliferation when compared to pure gelatin
and PCL scaffolds. Thus, the PCL-gelatin scaffold reported in our work has major prospective in the area of
wound dressings, treatment of diabetic foot ulcers, implants and transdermal drug delivery.
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| Keyword:
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Electro-spun scaffolds, PCL, gelatin, and F11 fibroblast cells
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| EOI:
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| DOI:
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| Download:
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