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Phenotypic and Genotypic Identification, Antibiogram of MRSA Isolated from Patients in Wasit city
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| Author:
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MUSTAFA K. QASIM, ZAINAB N. AL-SAADI, JASSIM FATEHI ALI
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| Abstract:
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Staphylococcus aureus is a common bacterium in both hospitals and society, and is now becoming more virulent and resistant to antibiotics. Since the 1960s, MRSA bacteria have appeared, spread globally and become the leading cause of bacterial infection in both health care centers and community environments.
The study started from November 2018 to April 2019, and 809 samples were collected from the patients from nose, ear, tonsils, pus, urine, and wounds) at Al-Kut Hospital for Women and Children, Al-Zahra Teaching Hospital, Al-Karama Teaching Hospital, and various medical clinics in Wasit city. Isolates were identified according to their phenotypic characteristics, biochemical tests, and API Staph system kit as well as to Vitek 2 system . The results showed that 208 of tested isolates identified as Staphylococcus aureus. MRSA isolates were detected phenotypically via testing the resistance of isolates to methicillin which was done via conducting sensitivity test for both cefoxitin and oxacillin, the results appeared that out of complete of 208 isolates, 78 isolates were resistant to each antibiotics and recognized as MRSA. Antibiotic sensitivity test of MRSA isolates toward 16 types of antibiotics has performed with Vitek 2 system, results showed that 100% of isolates were resistant to the penicillin G, cefoxitin and oxacillin (substitutes for methicillin). Isolates gave different ratios of resistance to the rest of the antibiotics depending on the type of antibiotic. Futhermore, isolates showed a high sensitivity towards linezolid (100%) and ciprofloxacin (93.7%) and gentamicin (93.9%). For molecular confirmation monoplex PCR technique has used to detect MRSA gentotypically, where mecA gene has detected in 50 isolates of phenotypically identified MRSA. The results were identical to the cefoxitin and oxacillin test for MRSA screening.
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| Keyword:
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Staphylococcus aureus, MRSA, Antibiogram , Patients , mecA gene.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP1.275
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| Download:
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