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Syndecan -4 and Alkaline Phosphatase Enhancement by Local Application of Exogenous Growth Factors on Traumatic Pulp of Osteoporotic Rats: Immuno-Histochemical Analysis
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| Author:
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ATHRAA Y.AL-HIJAZI, LOBNA K AL-KHAFAJI, NOOR NATIK RAHEEM
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| Abstract:
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Background: Osteoporosis is a disease associated with different risk factors, and many evidences suggest that it may be associated with the health of the oral cavity, including teeth and alveolar bone. In order to clarify the role of application of bone morphogenetic protein 2 (BMP2) and transforming growth factor beta 1 (TGF-ß1) in pulp repair for osteoporotic rat, we investigated their effects on the expression of Syndecan -4, alkaline phosphatase (ALP )and Collagen I by dental pulp cells of normal and osteoporotic rats.
Methods: Twelfth female rats (6 normal and the other 6 were osteoporotic rats), their maxillary anterior teeth have subjected to a mechanical traumatized pulptomy, where the left tooth has speared without treatment while the right tooth capped with application of 0.5 µl of BMP2 and 0.5µl of TGF ß1. Immuno-histochemical evaluation of Syndecan -4, ALP and Collagen I were estimated during the healing period in the examined pulp cells.
Results: Immuno-histochemical examination of tooth with pulptomy capped by BMP-2&TGF ß1 for both normal and osteoporotic rat revealed an increment in the expression of Syndecan -4 and ALP, with a significant differences value in comparison to control, while a non- significant difference was recorded for the collagen I.
Conclusion: The expression of Syndecan -4 and ALP in the traumatic pulp of osteoporotic rats could be affected by the local application of exogenous growth factors.
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| Keyword:
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BMP-2, osteoporosis, pulptomy, ALP, Syndecan-4, Collagen I, TGF ß1
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP1.251
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| Download:
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