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Molecular Identification and Prevalence of Some Virulence Genes among Pseudomonas aeruginosa Isolated from Iraqi Patients
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| Author:
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SANA MH AL-SHIMMARY
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| Abstract:
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Genomic variation of Pseudomonas aeruginosa, an opportunistic pathogen, play dramatic impacts on its ability to cause infection. It was predicted it's virulence depend on its genomic content. This study focused on prevalence of some main virulence genes of one hundred seventy three of P.aeruginosa isolated from different diseases including wounds, burns, keratitis, otitis and diabetic foot ulcers between September 2018 to November 2019 from hospitalized patients in Baghdad. In this study, it was used duplex and multiplex PCR in detection of virulence genes which are plcN, algD, lasB, exoS, plcH, respectively. The prevalence average of studied genes in the burns isolates was; algD(93.5%), plcN(82.6%), lasB(95.6%), exoS(80.4%) and plcH(98%);from wounds isolates was; algD(81.5%), plcN(87%), lasB(87%), exoS(63%) and plcH(94.7%);from keratitis isolates was; algD(25%), plcN(75%), lasB(91.6%), exoS(12.5%) and plcH(71.4%); from otitis isolates was; algD(14.3%), plcN(75%), lasB(89.2%), exoS(17.8%) and plcH(71.4%) and from diabetic foot ulcers isolates was; algD(70.2%), plcN(83.8%), lasB(94.6%), exoS(75.6%) and plcH(89.1%). The higher prevalence gene in burns and wound is pclH while in otitis and diabetic foot ulcers is lasB as well as in keratitis isolates lasB and plcH gave the higher rate equally. This finding show there are an important correlation between the virulence factors and the infections' site by this bacterium so they represent beneficial markers in diagnosis of clinical strains of P.aeruginosa.
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| Keyword:
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P. aeruginosa, virulence genes, duplex PCR, multiplex PCR
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP1.237
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| Download:
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