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In vitro antioxidant activity, total phenolic and flavonoid contents of aqueous and alcoholic extract of bilwadi agad
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| Author:
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KUMARI ANUPAMA, TIWARI R.C, SHARMA VED BHUSHAN, TIWARI SHASHIKANT, BHUTIANI RAKESH
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| Abstract:
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Introduction: The present study was undertaken to explore the antioxidant properties of Bilwadi agad. Bilwadi agad is one such formulation mentioned in classics which is the first drug of choice in acute toxicity conditions. The ingredients present in bilwadi agad are also known for their rasayana karma. The primary objective of this study was to evaluate the antioxidant effect of Bilwadi agad.
Methods: To fulfil the aim of the study two extract i.e aqueous and alcoholic extract of bilwadi agad were prepared and analysed in laboratory using different concentrations (5%, 10%, 15%) of extract. Aqueous and Alcoholic Extract of Bilwadi Agad had been prepared by reflex extraction method. Total phenolic content, DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay and total flavonoid content of bilwadi agad were determined.
Results: The results obtained during course of study were found significant, which showed antioxidant property of bilwadi agad. The obtained values clearly indicates that Bilwadi agad with both the extracts shows antioxidant activity or percentage inhibition of free radicals. The alcoholic extract showed more significant result in comparison to aqueous extract, it can be stated that more the concentration of extract more will be antioxidant property.
Conclusion: Present study has confirmed the antioxidant potentials of our preparation supporting its application as preventive remedy for various autoimmune and degenerative disorders. Finally it may be concluded that further study is required to isolate and identify the pure moiety responsible for antioxidant action of drug.
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| Keyword:
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antioxidant. alcoholic and aqueous extract, bilwadi agad, DPPH, phenolic content, flavonoid content, Rasayana.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP1.184
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| Download:
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