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DNA fingerprinting of the uropathogenic Escherichia coli clinical strains using ERIC1b-PCR, and PCR detection of Shiga like toxin (stx), espB, and EAF virulence genes
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| Author:
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, BAIDAA RASOUL DAKHIL, JAAFAR GH.ALOMAIRI , ABDULAZIZ SALIH ABDULAZIZ
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| Abstract:
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The current work does highlight the DNA fingerprinting of ten uropathogen clinical strains collected from patients suffered from urinary tract infections (UTIs) using: ERIC1b-PCR and PCR detection of stx-1, stx-2, and eae virulence genes. Ten E.coli out of sixty uropathogen clinical strains were isolated from patients suffering from UTIs after culturing the urine samples onto Eosin Methylene Blue, blood agar, and MacConkey, plates agar based on the distinctive colonial morphology of E.coli. Results of API20E system did confirm the identity of the uropathogen ten clinical strains as E.coli. ERIC1b-PCR did show a high potential in discrimination of the ten clinical strains into three groups with respect to their DNA banding patterns. The frequency of occurrence of the three virulence genes stx, espB, and EAF among the ten E.coli clinical strains under study was 100%; inferred from the resulting banding profile of PCR partial amplification with 399, 518, and 633 bp. The present data would underpin the potential of ERIC1b-PCR for strict differentiation among the uropathogen E.coli clinical strains.
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| Keyword:
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E.coli; UTIs; ERIC1b-PCR; stx, espB, and EAF
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP3.116
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| Download:
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