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Osteoconductivity evaluation of biomimetic hydroxyapatite coated PEEK dental implants: in vitro and in vivo histological study in a sheep model
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| Author:
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, ABDULJALEEL AZAD SAMAD, REIADH KAMAL AL-KAMALI
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| Abstract:
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In this study, osteoconductive activities of pure and hydroxyapatite (HA)-coated PEEK implants were examined in vitro and in vivo in male sheep. The uncoated and coated PEEK implants were evaluated in vitro (Cytotoxicity using MTT assay, Alkaline phosphatase activity (ALP), Osteocalcin production) and in vivo (Histological analysis using H&E staining, Cell count).
The MTT assay study of cultured fibroblasts on pure PEEK as well as HA-coated PEEK showed that biomimetic coating increased the prolifration of the cells. Studies of cultures of MG63 cell lines at three intervals of 24h, 48h and 1 week on implants showed that expression of ALP protein in one week (81.5 ± 3.02), 48 h (73.3 ± 4.15) and 24 h ( 62.01 ± 9.45) compared to the control group (31.79 ± 2.15) significantly increased (P <0.001). Studies of cultures of MG63 cell lines at three intervals of 24h, 48h and 1 week on implants showed that osteocalcin protein expression was significantly increased in one week (87.55 ± 3.4), 48 h (79.3 ± 5.2) and 24 h ( 73.48 ± 6.3) compared with the control group (36.94 ± 5.15) (P <0.001). Examination of histological sections of bone samples showed that during bone healing cellular proliferation reached its peak during the first-week post-injury followed by the maturation of cellular components and endochondral ossification which occurs near the end of the second week. The number of Osteocytes and osteoblasts as mean values increased during the transition from the 1, 2 to 4 and 8-weeks of healing intervals among all groups. While Osteoclast cell count in treatment groups decreased over time. In third week post-injury, the osteoblastic activity has been shown to gradually decrease and woven bone was replaced by lamellar bone. In damage area mostly fibrosis tissue have filled empty spaces and a little immature bone in some area near the native bone have produced. The border between the healing point and the normal bone area is clearly visible, but disruption was seen in fibrosis tissue near damage area.
In conclusion, the biomimetic coating of dental PEEK implants with bioactive HA significantly improves their clinical efficiency and overcomes the common limitation dental implants which is implant loose.
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| Keyword:
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PEEK; Biomimetic Hydroxyapatite Coating; in vivo; in vitro; histopathological analysis.
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP3.108
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| Download:
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