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Phylogenetic Background, biofilm and antibiogram profile of E.coli Isolated from Raw Milk Soled in The Local Markets at Al-Nasiriyah City- Iraq
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| Author:
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MUSLIM DHAHR MUSA, RABAB SHAKER SALMAN, ABBAS DAKHEEL MUTAR
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| Abstract:
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Current study was aimed to evaluate the potential risk of raw milk consumption that sold at local market to publichealth. Toachieve this mission the presence of E.coli was investigated as hygienic, quality indicator of raw milk also,phylogrouping, biofilm production ability and antibiogram was employed. Thus 54 raw milk samples were collected from two levels (markets and farms) at Al –Nasiriyah city south of Iraq, in period from January to March 2018. Traditional cultural and biochemical methods were used to isolate and identify E.coli, while molecular method ( Cleremont method) was used to determine phylogenetic groups. E.coli was detected in 15 samples (27.8%), recovery percentage was significantly higher (40.7%) at market than farm (14.8%). Phylogenetic analysis revealed that all isolated strains were belong to two phylogroups;A (53.4%) and B2 (46.6%). According to the source of isolation phylogroup B2 was found only at market level, while phylogroup A was found in both market and farmlevel. This study found that most strains of phylogroup B2 (83.4%) were strong biofilm producer and harbor a potential antibiotics resistance to commonly used antibiotics, as determine in this study the poor hygienic practice of entire process beginning from milking through transporting to selling at market was responsible of contamination of milk with pathogenic strains that belong to phylogroup B2 which is mostly probably come from human source .In conclusion the raw milk constitute a serious public health threat to people who consumed raw milk
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| Keyword:
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E.coli , Raw milk, Phylogenetic grouping, Antibiogram, Biofilm, Iraq
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