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Detection of biofilm genes within staphylococcus aureus from gingivitis cases
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| Author:
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SIRWAN AHMED RASHID, SAWSAN MUHAMMED SORCHEE
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| Abstract:
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In this study fifty sample of patients diagnosed clinically as having gingivitis were collected from
laboratory of Faculty for Dentistry in hawler medical university during the period of November 2018 to
February 2019. Eleven isolates of staphylococcus aureus bacteria were identified by using traditional culture
method and vitek 2. The susceptibility of staphylococcus aureus isolates to different antibiotics was examined.
Vancomycin was the most effective antimicrobial agents against staphylococcus aureus isolates (100%),
Imipenem (81.8%), Tetracycline (72.7%), Amikacin (72.7%), Chloramphenicol (63.4) and Ciprofloxacin (54.5%)
and many of isolates showed resistance to Ampicillin (81.9%), Clindamycin (63.6%) and Erythromycin
(63.6%).In addition, estimate the ability of staphylococcus aureus to produce biofilm were studied using
congo red agar method , and the results showed that 10 (90.9%) of the isolates are biofilm positive and
biofilm producers and one isolate were negative biofilm.As well as the results of molecular analysis by using
PCR showed that isolated staphylococcus aureus were carried biofilm genes icaC (100%), icaD (100%), cna
(90.9%) and fnbA (100%).
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| Keyword:
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biofilm genes , staphylococcus aureus , gingivitis cases
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| EOI:
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| DOI:
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| Download:
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