|
Comparative study for the frequency of papC, fimH and iucD genes in E. coli isolates from UTI and vaginal infections
|
|
| Author:
|
, ABDUL-KAREEM SALMAN AL-YASSARI, JAAFAR GH. ALOMAIRI, SAAD ABDUL AZEEZ ATIYAH
|
| Abstract:
|
Genito-urinary tract infections in women represents an important medical problem around the world and they are leading cause of morbidity especially in pregnant women, E. coli is a common cause of these infections. The study aimed to compare between E. coli isolates from genito-urinary tract infections in there carrying of some virulence genes. A complete of 42 E. coli isolates involved in current study involve 21 isolates from vaginal infections and 21 isolates from UTI, the frequency of virulence genes papC, iucD and fimH was detected by polymerase chain reaction using specific primers. The results revealed the frequency of papC, iucD and fimH genes were (33.3, 16.7 and 31.0) respectively with significant differences under p value < 0.05, the E. coli isolates from UTI showed 38.1, 14.3 and 33.3 % positive for each above gene, While the isolates from vaginal infections give a positive amplification results for papC, iucD and fimH genes in the percentage 28.6, 19 and 28.6 %, respectively with significant differences under p value < 0.05. But, there are no differences in the expression of these genes between the isolates from the above sources. Also, the results show correlation between the frequency of fimH from vagina with fimH of UTI and iucD from vagina with p-value 0.04 and 0.02 respectively. In conclusion, the presence of papC, iucD and fimH genes have the same percentages nearly between isolates from UTI and vaginal infections with the dominancy of papC and fimH when compared with iucD gene and this study suggestive that these E. coli isolates may came from the same source.
|
| Keyword:
|
E. coli, virulence factors, Genito-urinary tract infections
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2020.SP2.570
|
| Download:
|
Request For Article
|
|
|