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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Bioactive Fraction of Galangal Rhizome (Alpinia galanga): Determination of Acetoxychavicol Acetate Concentration By HPLC and Cytotoxyc Activity on T47D Cancer Cell Line

Author: MUHAMMAD DA’I, SINDYA SHOFI MUNIRA , AZIS SAIFUDIN, ANDI SUHENDI
Abstract: Background Galangal Rhizome (Alpinia galanga) has the semi polar main content of 1'-acetoxychavicol acetate (ACA), which is potential to be a cytotoxic agent in T47D cells. The present study has not evaluated the cytotoxic activity of the galangal fraction against cancer cell line T47D more. This study aimed to determine the cytotoxic activity of T47D cells and the concentration of ACA contained in ethanol extract, ethanol-water fraction, ethyl acetate fraction, and n-hexane fraction of galangal rhizomes. Materials and Methods The ethanol extract of galangal rhizome was fractionated with liquid-liquid partition method. Cytotoxic activity was carried out by MTT assay and ACA determination using the RP-HPLC method with the mobile phase of acetonitrile: methanol (75:25), flow rate of 1 mL/minutes, and wavelength of 253 nm. The calibration curve of ACA obtained was y = 2932.8x - 4848.4 with the correlation coefficient (r) = 0.999. Results The results showed the greater cytotoxic activity of ethyl acetate fraction compared to other samples with IC50 values of 100.46 µg/mL and ACA content of 4.49 ± 0.09 mg/mL. Thus, the relation between cytotoxic activities in the four samples is directly proportional to the ACA levels.
Keyword: galangal rhizome, 1’-acetoxychavicol acetate, cytotoxic, T47D, HPLC
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