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Inhibiting Protein Tyrosine Phosphatase 1B enzyme Induced Apoptosis In HCT 116 Colon Cancer Cell Line.
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| Author:
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LUBNA A.MAHDI, SAMEER H. ABBOOD AL-REKABI, TALIB H. KAMOONA
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| Abstract:
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Colorectal cancer (CRC) is third most common cancers and second death-causing cancer around the world. One of the factors that stimulate colon cancer cells to proliferate and invade other tissues is the disturbed balance between proteins phosphorylation and dephosphorylation by kinases and phosphatases. For a long time researches had focused on the potential effect of kinases on oncogenesis, while phosphatases role was assumed to be tumor suppressive. This principle, however; had changed in last few years when researches showed that phosphatases might also stimulate growth of cancer. One of these enzymes is protein tyrosine phosphatase 1B (PTP1B). Both PTP1B enzyme expression and activity are enhanced in CRC cells as compared with normal tissues. Knocking down of PTP1B in CRC cell lines resulted in less a phenotype with less invasion and proliferation capabilities. Here we report that inhibition of PTP1B in colorectal cancer cell line HCT 116 by using claramine trifluoroacetate (SML 1545) solution resulted in increased apoptotic cells in treated samples compared with those which were untreated.
Objectives: This study had been conducted to clarify the possible apoptotic effect of claramine on colon cancer cells.
Materials and Methods: HCT 116 colon cancer cell line seeded on a 96-well plate was exposed to serial dilutions solutions of claramine trifluoroacetate solution. The plate then was incubated at 37ºC for 24hr. The apoptosis was estimated by measuring Annexin V flowcytometry and apoptosis inducing factor ELISA Assay.
Results: Both Annexin V and AIF assays showed that claramine significantly increased apoptotic cells in treated HCT 116 cells compared with control HCT 116 cells.
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| Keyword:
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Colorectal cancer, protein tyrosine phosphatase, claramine trifluoroacetate, annexin V, apoptosis inducing factor.
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP2.542
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| Download:
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