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Evaluation Of Antibacterial Activity Of Curcumin Assisted Silver Nanoparticles On Oral Pathogens
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| Author:
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SREEDEVI DHARMAN, MARAGATHAVALLI.G, RAJESHKUMAR.S, KARPAGAVALLI SHANMUGASUNDARAM
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| Abstract:
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Oral bacteria colonize and cause pathology chiefly in the oral cavity, which is a source of systemic diseases such as bacteremia and septicemia. With Widespread use of Antibiotics, bacteria developed resistance to almost all synthetic drugs. Reduction in bacterial resistance and adverse effects of drugs is achieved by an alternative approach such as phytochemicals via green synthesis nanotechnology that equally combats the pathogenic bacteria. This research aimed to assess the antibacterial activity of curcumin assisted silver nanoparticles on oral pathogens. Curcumin were used to reduce silver nitrate into silver atom and synthesize biocompatible silver nanoparticles. They were analyzed by UV-visible absorption spectroscopy(UV-Vis) and Transmission electron microscopy(TEM) . Antibacterial activity is evaluated against oral pathogens such as Gram positive bacteria - Staphylococcus aureus, Streptococcus mutans, Gram negative bacteria - E. coli , Enterococcus sp. Color alteration from initial yellow solution to dark brown within 1hour of reaction time and the absorbance peak at 430 nm confirm the silver nanoparticles formation. TEM showed curcumin assisted silver nanoparticles were spherical and triangular in structure with measurement range from 5-70 nm. Curcumin assisted silver nanoparticles showed higher antibacterial activity towards oral pathogens such as S. mutans, medium ranged antibacterial activity towards S. aureus, E. coli and Enterococcus sp in 50-µg mL-1.Curcumin assisted silver nanoparticles proves to be an effective broad spectrum antibacterial agent, which improves its scope in potential therapeutic application of various oral infectious diseases with reduced side effects.
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| Keyword:
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Curcumin, antibacterial, silver nanoparticles, Green synthesis, UV–vis spectroscopy, TEM, Laboratory Research
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP1.490
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| Download:
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