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Isolation and characterization of Endophytic Actinomycetes Streptomycets sp. from some local algae isolates in Al-Islah marshes of Thi-Qar Provence southern of Iraq .
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| Author:
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NAFAA FARHAN MUFTEN, EZAT HUSSIAN MEZAL, AHMED SHAKER AL-ASHOOR
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| Abstract:
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The current study, which continued to isolate and identify Endophytic Actinomycetes Streptomycets sp. from local algae isolates ( chlorophyta ), (Chladophora sp. and Ulothrix sp.) Algae isolate from different region of Al- Islah marshes of Thi-Qar provence southern of Iraq. The Endophytic Actinomycetes were isolated by Actnomycetes Isolation Agar (AIA) plate method after surface sterilization.The potent Endophytic Actinomycetes were characterized by morphologyical methods consist of macro and micro-scopic examinations. The mycelium structure, color and arrangement of conidiophores were observed by the oil immersion ( 100 X). Typical colonies from actinomycetes isolation agar ( AIA) plates were identified and performed, the results were recorded. Various biochemical testes performed for the identification of potent isolates such as : Gram staining, IMVIC test, urease test oxidase , H2S production test and catalase test . The results showed that from A total of 13 Entophytic actinomycete (EA) Spp. ther was 4 isolates had shown varying morphological, physiological and biochemical characteristics. All these results with Bergeys Manual of Determinative Bacteriology and the organisms were identified. All the isolates were identified to belong the Entophytic actinomycetes Streptomycets sp. Identification of the strain was carried out by employing the taxonomical studies including the 16S rRNA sequence, From the results, it was suggested that use of these isolates to produce new antibiotics against pathogenic bacteria, fungi and other pathogens.
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| Keyword:
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Endophytic Actinomycetes, 16S RNA, Biochemical, Characteristics, Thi-Qar-Iraq.
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP2.509
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| Download:
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