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The effect of induced oxidative stress by Short and long –terms Exposure to potassium sorbate (E202) as a food additive on the Female Reproductive System of Wistar Rats
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| Author:
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ALAA MOHAMMAD HASSON
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| Abstract:
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Background: Potassium sorbate is the potassium salt of sorbic acid .It is considered the safest preservative as it is widely used in food preservative, however, it is not without side effects.in vivo and in vitro studies investigated that PS induced oxidative stress, which leads to tissues damage.
Aim: This study was conducted using Female rats as a typical for the mammals' animals to determine the levels of oxidative stress biomarkers in serum and ovary , reproductive hormones and histological change that can cause after Exposure to potassium sorbate as a food additive in their diet.
Methods: In present study ,60 adult virgin Females rat . It was divided into two secondary experimental groups represent a short and long – terms studies , each included 30 Females rat. Each secondary experimental group divided into three subgroups, each subgroup involved (10) rats. Females rats of control subgroups fed normal diets without adding potassium sorbate, the females rats of first and secondary subgroups (G1 and G2) feeding daily with PS in their diet at per cents 5% and 10% respectively .At the end of each term, females rats were sacrificed and blood samples were collected to estimated levels of oxidative stress biomarkers (MDA and CAT activity) and reproductive hormones (FSH,LH,PROG and E2) in serum .The ovaries, oviducts and uteri for detection of histological changes in reproductive organs, in addition to determine the levels of MDA and CAT activity in ovaries.
Results: Consumption of PS at per cents 5% and 10% in the long – term causes a significant rise of MDA and significant decline of CAT enzyme activity in plasma. In ovaries tissues, MDA levels increase at per cents 5% and 10% in both periods short and long – terms. While the decline of CAT enzyme activity noted at 10% of PS in the long – term only. This study confirmed found a positive significant correlation between MDA of plasma and ovaries in the long – term .Also, there was a positive significant correlation between CAT activity of plasma and ovaries in short and long – terms. The levels of hormones FSH, LH, PROG and E2 decreased significantly at 10% of PS in the long – term study. While a high level of plasma E2 is observed at 10% of PS in the short– term study. The histopathological change observed in studied tissues especially females rats' that consumption of PS at per cent 10% for a short and long – terms, including, follicular atresia in oocytes of rat's ovaries, inflammation in oviducts and degeneration of endometrium in the uterus of females rats'.
Conclusions: in this study, can conclude according to the obtained results that potassium sorbate can induce oxidative stress by elevation of MDA concentration and dimension of CAT enzyme activity that reverse negatively on sex hormonal and reproductive tract.
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| Keyword:
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Potassium sorbate, MDA, CAT, FSH, LH, PROG, E2. Ovary, oviduct, uterus.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP2.503
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| Download:
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