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Association of IL-28B Gene Polymorphism and Serum Level with Hepatitis C Virus Infection in Iraq
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| Author:
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NAWAR B.ABDULSAHIB, HASSAN M.NAIF, SAFAA ABDULKAREEM ALWAYSI
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| Abstract:
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Hepatitis C virus (HCV) infection is a leading global health problem and 130-150 million individuals are chronically infected with HCV, which progress to cirrhosis or hepatocellular carcinoma. A total of 120 subjects were used for assessing the correlation between gene polymorphisms and serum level of IL-28B with the susceptibility to HCV infection in Iraqi patients. Gene polymorphism was detected by RFLP PCR restricted with BseMI and BsrDI. The serum level was assessed by ELISA. The gene polymorphism distribution of IL-28BC/T (rs12979860) and IL-28T/G (rs8099917) appeared in both patients and control groups in three genotypes with variable ratios. The allele frequencies and genotype distribution of CC and CT did not show differences, but the TT showed significant differences (P=0.047) between HCV infected patients and healthy controls. Regarding the second polymorphism of IL-28T/G, the GG distribution and allele frequency of G and T had no major differences. On the other hand, the TG and TT distribution had significant differences (P=0.0089 and 0.0251, respectively) between HCV infected patients and controls. Antiviral treatment response against HCV had no significant influence on either allele or genotype distribution of both IL-28 SNPs. The circulating levels of IL-28B in patients was higher by three folds than its value in the control group. These results indicated that both IL-28B mutant SNPs had significant correlation with the susceptibility to HCV infection. Serum level of IL-28B showed protective effect to HCV infection.
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| Keyword:
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Hepatitis C virus, IL-28B, RFLP, PCR, Polymorphism, Cytokine. Iraq
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP2. 491
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| Download:
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