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The Effect of Antioxidant Cysteamine With Ascorbic Acid On In Vitro Fertilization in Cows
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| Author:
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HUSAMALDEEN A. ALSALIM, IHSAN A. HABEEB, HAIDER R. ABBAS
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| Abstract:
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Oxidative stress (OS) has been recognized as an effective reason for decreasing the outcome of assisted reproductive technology (ART). The supplementation of antioxidants in culture medium has been used widely to improve the in vitro fertilization (IVF) in many species. This study was conducted to evaluate the effect of cysteamine (CYS) or ascorbic acids (AA) (either in combination or individually) as antioxidants in all stages of bovine IVF, particularly on in vitro oocytes maturation, cleavage rate and subsequent development of blastocysts. We found that adding of 100 µM CYS or 200 mM AA significantly (p<0.05) improved the bovine oocytes maturation compered to control group, while using of CYS plus AA didn’t improve the oocytes maturation compared to CYS or AA groups. The same dose of CYS or AA (either in combination or individually) didn’t improve the cleavage rate but CYS and CYS plus AA significantly (p<0.05) induced the early cleavage (= 24 h after IVF) and formation of 2 cells embryos compered to AA and control groups. The supplementation of CYS or AA (either in combination or individually) to culture medium significantly (p<0.05) improved the blastocyst rate after IVF and also prolonged the viability of blastocysts till day ninth from IVF in CYS and CYS plus AA groups compared to AA and control groups. In conclusion, the supplementation of in vitro culture medium with antioxidant CYS or AA improve the bovine IVF and subsequent production of blastocyst compared to control group but the combination between them did not improve bovine IVF compared to control group.
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| Keyword:
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antioxidant, cysteamine, ascorbic acid, in vitro fertilization
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP2.459
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| Download:
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