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Analysis on Restriction Fragment Polymorphism and Determination of Genetic Diversity of Mitochondrial DNA form SEPIA Species
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| Author:
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AKILA , MALINI EVANGELINE ROSE, DR G SRAVANI, DR PRATYUSHA
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| Abstract:
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The nucleotide divergence between sequences of one gene or a portion of a gene from the mitochondrial genome can be analyzed phylogenetically. Mitochondrial DNA (mtDNA) sequence diversity is utilized in several trainings to differentiate between organisms and to establish relationships between them. Nucleotide differences between individuals can be identified by using constraint endonucleases that cut the DNA at detailed recognition orders. Fragments of different sizes are generated when individuals differ in their DNA sequence; these are called restriction fragment length polymorphisms (RFLPs). RFLPs of mtDNA have proven to be useful for determining relatedness among the closely related organisms. The findings of the existence of mitochondrial DNA in all animals and plants and the isolation and molecular analysis of the same have emerged to answer many intra-species relationships between different organisms
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| Keyword:
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mtDNA, Sepia, RFLP, Electrophoresis
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP1.465
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| Download:
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Request For Article
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