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Estimation of Chlorthalidone API Tablet for Stability Indication and Validation using RP-HPLC Techniques
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| Author:
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PARTHASARATHY M.P, SARAVANAN V, DR M SMITHA, DR GUNASAGAR DAS
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| Abstract:
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A fast, exact, easy, sensitive, & precise opposite stage fluid chromatographic strategy is established for Chlorthalidone determination in drug & bulk dose form dosage structure. The chromatographic strategy was normalized utilizing Develosil ODS HG-5 RP C18, 5µm, 15cmx4.6mm i.d. column with UV discovery at 245 nm and 0.1% Orthophosphoric acid: Acetonitrile: Methanol (12:18:70 v/v/v) proportion at a stream pace of 1.0 ml/min. The proposed strategy was effectively applied to the assurance of Chlorthalidone in mass and drug measurement structure. The technique was direct over the scope of 0-14µg/ml. The recuperation was in the scope of 98% to 102% and restrict of identification was discovered to be 0.08 µg/ml and measurement was discovered to be 0.24 µg/ml. Distinctive systematic execution boundaries, for example, exactness, precision, cutoff of location, breaking point of evaluation and power were resolved by International Conference on Harmonization (ICH) rules
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| Keyword:
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RP-HPLC, chlorthalidone, ICH guidelines, limit of detection (LOD), Limit of Quantitation (LOQ), Signal-to-noise ratio (SNR)
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.SP1.459
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| Download:
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