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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Detection of Theileriaannulata isolated from cattle using Real-Time PCR

Author: QASSIM AMEER, NAWRAS ABDUL BARI MADLOL, NAER ABDULBARIMADLOOL AL-KAABAWI
Abstract: This study aimed to molecular investigation the prevalence of Theileriaannulataparasite that isolated from blood samples of cattle. A hundred of blood sampleswascollected randomanly from cattles ofthe middle area of Iraq (Babylon province). Samples were submitted to blood DNA extraction assay. Nano drop spectrophotometer and QRT-PCR with TaqMan methods were involved for the surface antigen Tams1detection of Theileriaannulata. Real- Time PCR results showed only 16 samples were positive out of 50 blood samples with a percentage 32%. The threshold cycle (CT value) of these positive samples were ranged from CT: 24.29 to CT: 35.69. The Real-Time PCRefficiency was validated using standard curve method, and the efficiently was approximately (99.9%).Surprisingly, the overall results from current study showed there were no association between ages, gender. Infectionsdistribution between (1-12month) (28.57%), 1-2 year (31.57%) and 3-10 year (29.4%).Additionally, the ratio of the infected females at age of 1-2yearswere twice more than the ratio of infected males.
Keyword: Theileriaannulata, Cattle, PCR
DOI: https://doi.org/10.31838/ijpr/2019.11.02.081
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