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Anticarcinogenic ,immune modulatory and Antibacterial effect of L-Argininedeiminase ADI against S. epidermidesCoNS mammary gland infection model
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| Author:
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LIKAAHAMIED MAHDI, BATOOLABD AL AMEERBAQERAND, IBTESAMGHADBANAUDA
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| Abstract:
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The study sought to purify a novel L- Arginine deiminase enzyme (ADI) from L.pentosusand studying the effect of L.
pentosusand ADI againstmultidrug-resistant S. epidermidesCoNS isolatesin vivoand in vitro. ADI from
Lactobacilluspentosuswas extracted,purified andcharacterized.the optimum pH activity was found to be at pH 6.5
,ADI activity increased when the enzyme was incubated with MnCl2, FeCl2, MgCl2 and HgCl2 with 5 and 10 mM
as ADI activitors, which may indicate that this enzyme may exhibit as a metaloenzyme. The molecular weight of
purified ADI was 178000 Dalton . The antibacterial activity of purifiedADI was higher than crude (P<0.05). while there
isan improvement of S. epidermidesinfection in mammary gland ADI treated groups(P<0.05). Analysis of serum IL-10
and IL-12 levels revealed ADI hasprophylaxis effect. In conclusion, ADI is protein in nature,This enzyme that
hydrolyze arginine to generate energy in many microorganisms and it has a potent anticancer activities and can halt
growth of solid tumors catalyzes the irreversible hydrolysis of arginine,Lactobacillus pentosusand ADI hasanti-S.
epidermides activity, immunomodulatory by increasing pro-inflammatorycytokines and - S. epidermidesin mammary
gland infection model in vivoand in vitro.
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| Keyword:
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Lactobacillus pentosus, L- Arginine deiminase ADI, Antibacterial, anti-S. epidermides activity, immunomodulatory, cytotoxicity.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.02.080
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| Download:
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