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Characterization and Evaluation of Antimicrobial and Cytotoxic Activities of Glycine max Methanol Extract
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| Author:
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HEBA ABDEL-HADYA, EMAN AHMED EL-WAKILA, SAMI MOHAMED NASRB
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| Abstract:
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Glycine max seeds use in many types of food in Egypt and all over the world. Also, it has many pharmacological
properties. This study aimed to evaluate cytotoxic and antimicrobial activities of the plant extract on murine spleen
cells and hepatocellular carcinoma (HepG2 cell line) as well as GC-MS analysis. The seeds were extracted with 85%
methanol and tested on murine spleen cells and HepG2 cell line with different concentrations. Also, antimicrobial
activity of the extract was studied by mean inhibition zone. The outcomes proved that the cytotoxic activity of the
plant on murine spleen cells is very weak (380.94µg/ml) as well as on HepG2, it inhibits the cell viability% to 8.5%
after 24 hrs. (IC50 = 42.5µg/ml). Also, the plant showed good antimicrobial activity in a concentration-dependent
manner on fungi and bacteria (Gram positive and Gram negative). GC-MS analysis of bioactive compounds in the plant
extract revealed the presence of 11 bioactive compounds. The major bioactive compounds are 9,12-octadecadienoic
acid (Z,Z)-, methyl ester (41.24 %), hexadecanoic acid, methyl Ester (27.39 %), 9 octadecenoic acid (Z)-,methyl ester
(16.58%), methyl stearate (4.71%), ethyl linoleate (3.92%) and n-hexadecanoic acid (2.34%). According to these
finding, Glycine max methanol extract is potential as anticancer and antimicrobial agents that is may be due to the
identified bioactive compoundsby GC-MS analysis. Therefore, the phytochemicals of the plant may be responsible for
the various pharmacological actions.
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| Keyword:
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Antibacterial, Antifungal,GC-MS analysis, HepG2 cell line, Murine splenic cells, Soya bean
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.02.060
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| Download:
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