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Effect of culture media and growth hormones on callus induction in Crataeva tapia L.
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| Author:
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PRIYANKA SHARMA, AVINASH PATIL, DARSHANA PATIL
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| Abstract:
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Crataeva tapia L., is an important medicinal tree which is harvested in huge quantities by pharmaceutical companies (Family Capparaceae). This tree is mostly propagated by seeds but this propagation is highly impeded because of poor seed germination mainly due to presence of hard seed coat and heavy infestation by insects. Cultivation of the plant needs to be promoted to meet continuous demand for the raw materials. In the present study, a protocol for callus induction has been developed using leaf, stem, petiole and petiolule as explants from field grown plants of Crataeva tapia L. and inoculaed on MS, B5 and WPM media fortified with two PGRs, 2,4-D (0.5 - 2.5 mg/l), BAP (0.5 - 2.5 mg/l) and CM (5-15%) for callus induction.
Out of the three media used, best callus response was obtained on MS medium with leaf and stem as explants along with BAP (0.5-2.5 mg/l) and 2,4-D (0.5-2.5 mg/l) in combination with 10% CM. The data obtained from the present study may be used for micropropagation and in vitro biosynthesis of secondary metabolites of commercial significance using callus. This may help in reducing uncontrolled overexploitation of this tree species, thus conserving the plant in its natural habitat.
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| Keyword:
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Crataeva tapia L., callus culture, MS medium, 2,4-D, BAP, 10% CM.
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| EOI:
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| DOI:
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