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High Performance Thin Layer Chromatographic Determination of Ethinylestradiol and Levonorgestrel in Combined Tablet Dosage Form
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| Author:
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UPLOADED BY-ADMIN, PADMANABH DESHPANDE, SANTOSH GANDHI, NILESH GAIKWAD, VISHAL MHATRE, JITESH PARAB
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| Abstract:
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A simple, accurate and validated high performance thin layer chromatographic (HPTLC) method for determination of
Ethinylestradiol and Levonorgestrel in combined tablet dosage form has been developed. The mobile phase selected was n-
Hexane: Ethyl acetate: Triethylamine (8: 2: 0.5 v/v/v) with UV detection at 220 nm. The retention factor for Ethinylestradiol
and Levonorgestrel were found to be 0.19 ± 0.10 and 0.50 ± 0.12. The method was validated with respect to linearity, accuracy,
precision and robustness. Results found to be linear in the concentration range of 200-1000 ng/band for Ethinylestradiol
and 1000-5000 ng/band for Levonorgestrel. The method has been successfully applied for the analysis of drugs in pharmaceutical
formulation. The % assay (Mean ± S.D.) was found to be 98.82 ± 0.14 for Ethinylestradiol and 99.12 ± 0.11 for
Levonorgestrel.
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| Keyword:
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Ethinylestradiol, Levonorgestrel, High Performance Thin Layer chromatography, Tablet dosage form
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| EOI:
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| DOI:
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