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HPTLC fingerprinting of different extracts and estimation of phytoconstituents of Ajuga bracteosa
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| Author:
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SIDDHIBEN UPADHYAY, DR. V. C. JAIN, DR. U. M. UPADHYAY
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| Abstract:
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Ajuga bracteosa belongs to family Labiatae; commonly known as Neelkanthi; distributed in subtropical and temperate regions, was collected, identified and subjected to Successive extraction to prepare Petroleum ether, Benzene, Chloroform, Acetone, Ethanol and Aqueous extracts. Preliminary Phytochemical Screening was performed using chemical reagents to check the presence of 12 different Phytoconstituents in these extracts namely Fatty acids, Steroids, Cardiac Glycosides, Saponins, Phenolics, Flavonoids, Bitters, Anthraquinones, Alkaloids, Volatile principles, Tannins and few Carbohydrates. Fatty acids were found to be present in all the extracts. Volatile oils were detected in all the extracts except Aqueous extract. Similarly Anthraquinones were found to be present in all the extracts except Petroleum ether extract. Ethanolic extract appears to be containing almost all the phytoconstitutents. All the extracts were found to be devoid of Gums, Mucilage, Amino acids, Coumarin Glycosides and Cyanogenetic Glycosides. All these extracts were then subjected to Chromatographic separation using different solvent systems and the respective markers. The chromatograms obtained after HPTLC fingerprinting of these different extracts provided the most useful information for the standardization of Ajuga bracteosa. The corresponding peaks present in the chromatograms revealed the presence of several compounds in the extracts and also yielded the critical information of AUC as well as Rf value. The simultaneous application of Marker helped us to establish presence of a specific phytoconstitutent in the different extracts and it can be quantified also. Total Phenolics, and Gallic acids were quantitatively estimated to be 21.9 % & 0.4 % respectively in the Ethanol extract. Such finger printing is useful in differentiating the species from the adulterant and act as biochemical markers for this medicinally important plant in the Pharma industry and plant systematic studies.
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| Keyword:
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HPTLC, Fingerprinting, Ajuga bracteosa, Gallic acid
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