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The Protective Activity of Saccharum officianarum Against CCl4 Induced Hepatotoxicity in Rats
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| Author:
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UPLOADED BY-ADMIN, PATEL BA, PATEL JD, RAVAL BP, GANDHI TR
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| Abstract:
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ml/kg, i. p.) once daily for seven days. Saccharum officinarum juice (0.75ml/100gm, P.O.) was given for seven days.
Silymarin (200mg/kg, p. o) was used as a reference drug. Levels of different marker enzymes were estimated in serum. A
probe into the mechanism of action was attempted by estimating total protein, malondialdehyde and glutathione levels in
liver homogenates in order to evaluate the degree of lipid peroxidation. Histopathological studies were also done to confirm
the biochemical changes. In vitro, Hepatocytes of control group were exposed to CCL4, while cell suspensions were pretreated
with S. officianrum (1mg/ml) before exposure to CCL4 and standard group received silymarin (1mg/ml) before exposure
to CCL4. While in vitro model, estimation of percentage viability of hepatocytes was evaluated by trypan blue assay.
CCl4 produced significant changes in physical (increased liver weight and volume), biochemical (increase in serum alanine
transaminase, aspartate transaminase, alkaline phosphatase, direct bilirubin, total bilirubin and triglycerides), liver tissue
(decreased totoal protein, GHS and increased MDA), histological (hepatocytes damage) and functional (thiopentone induced
sleeping time) liver parameters. Treatment with S. officinarum stem juice significantly prevented the physical, biochemical,
histological and functional changes induced by ethanol in the liver, while in vitro model exhibited significant increase in
percentage viability of cells with preexposure to S.officinarum and Silymarin as compared to CCl4 control. Juice of S. officinarum
exhibited significant hepatoprotective action against CCl4 induced hepatic injury both in vivo and in vitro study.
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| Keyword:
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CCl4, hepatoprotective activity, juice, Saccharum officinarum, antioxidant.
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