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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Evaluation of Antibacterial activity of Essential oil of Trachyspermum ammi (Ajwain)

Author: MISS GOSWAMI, DR SREEMOYEE CHATTERJEE, MS. NANDINI GOSWAMI, MS. SHALINI KHANDELWAL
Abstract: Emergence of new antibiotics in the bio field is eradicating bacterial infections but at the risk of giving rise to new antibiotic-resistant bacteria. To overcome this unsustainable situation natural bioactive compounds which are traditionally used globally can prove to be a better alternative. Extracted essential oils have also been shown to contain biologically active constituents that are proven to be antibacterial agents. The present study was carried out to investigate the antimicrobial activity of this oil from Trachyspermum ammi (Ajwain) against four pathogenic bacteria (Gram positive bacteria Staphylococcus aureus and Gram negative bacteria Escherichia coli, Proteus vulgaris and Pseudomonas aeruginosa) following disc diffusion method. Different concentrations (2.5-320 µl/ml) of essential oil were used to determine minimum inhibitory concentration (MIC) of the oil. The essential oil was active against all tested bacteria and has shown comparable results when tested against standard antibiotics. The essential oil has shown concentration dependent antimicrobial activity. The highest significant antimicrobial activity with the best MIC value was produced against Escherichia coli .The MIC for Escherichia coli was 5 µl/ml and 10 µl/ml for Proteus vulgaris, Pseudomonas aeruginosa and Staphylococcus aureus.
Keyword: Antibacterial agents, Trachyspermum ammi, minimal inhibitory concentration, ajwain.
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