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Molecular Detection of Antimicrobial Resistance Genes in Urinary Tractional Infectious (UTI) patients In Babylon Site, Iraq
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| Author:
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ABBAS. GHALI.HAMED, ABBAS.K. AL-MANSOORI, MOHAMMED .TALIB. AL-TAMIMI
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| Abstract:
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Beta-Lactamase resistance among Enterobacteriaceae, especially in Escherich coli, is an emerging problem worldwide.
The present study focused on determining prevalence of (blaCTX-M, and blaSHV-5genes coding Beta-Lactamase
resistant in to the family Enterobacteriaceae via PCR technique. 30 clinical bacterial isolate were obtained from
Babylon teaching hospital for identification UTI Nosocomial infection patients. E. coli was the most common bacterial
strains from Beta-Lactamase isolates belonging to the family Enterobacteriaceae. The results of the present study
indicated that prevalence of Beta-Lactamase resistant Escherich coli isolates was from patients (2 to 18 years) since
that E. coli Beta-Lactamase genes were detected in some labs out of the 71.6 positive isolates. blaAMPC and bla TEM
were significantly prevalence in all bacterial isolates compare with blaCTX-M and blaSH-5 at p = 0.05. The
percentage of the prevalance of blaAMPC and bla TEM were 93.33 and 71.66respectively while the less percentage
were observed in blaSHV-5 blaAMPC 20% and bla TEM 46.66%.Virulence factors were also examined between these
genes and some other genes that were studied in the same laboratory by other group at the same university to
inspect the possible relation between virulence genes and antimicrobial resistance in some pathogenic bacteria.
Considering the fact that the presence of recognized infective determinants among clinical isolates may promote the
emergence of infections and persistence of pathogens. The results of virulence gene indicated that biofilm gene was
the most prevalence gene, where appeared in 95% of bacterial isolates following by iss gene 86.6% and fimH gene
85%.Current study showed a strong significant correlation between virulence and antibiotic resistance profile , where
the statistical analysis revealed that the person correlation was 0.957 between virulence and antibiotic s
determinants. Hence the establishing of the correlation between resistance and virulence profile could provide input
for the clinical evolution and of different bacterial infection rates recurrence.
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| Keyword:
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Beta-Lactamase, blaCTX-M, blaSHV-5
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.02.031
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| Download:
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